Inflammation is a complex disease which is associated with various symptoms. These symptoms are manged with non-steroidal and steroidal anti-inflammatory drugs, which lead to severe side effects on long-term. But natural product can be used as an alternative source with the discovery of new entities to be used as anti-inflammatory compounds to overcome this problem. The compound isolated by using the soxhlet apparatus and column chromatography. The objective of the present study is to evaluate the anti-inflammatory activity of isolated compound Phorbol from methanolic extract of W. floribunda Salisb for scientific validation of the folklore claim of the plant. The plant flowers is rich in antioxidants and has been suggested as a potential source of anti-inflammatory compounds.