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ISSN 2063-5346
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PHARMACOLOGICAL EVALUATION OF IN- VITRO AND INVIVO ANTI-RHEUMATOID ARTHRITIS ACTIVITY OF POLYHERBAL EXTRACT OF SELECTED TRADITIONAL INDIAN PLANTS IN WISTAR RATS.

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Rekha Gour1*, Dr. Patel Bavik Ambalal
» doi: 10.48047/ecb/2023.12.si5a.0112

Abstract

Arthritis is leading cause of disability in adults all over the world, limiting day-to-day activities for billion of people. WHO reports that 1% of the world population is affected by arthritis. The present study aims to evaluate the effectiveness of a polyherbal extract Imperata cylindrica, Abroma augusta, Feronia limonia and Abelmoschus esculentus for the treatment of rheumatoid arthritis by in-vitro and in-vivo methods. Materials and Methods: Poly herbal ethanolic extract of different fractions are used to investigate in vitro by human red blood cell (HRBC) membrane stabilization assay, inhibition of protein denaturation assay using egg albumin, and inhibition of protein denaturation assay using bovine serum. Furthermore, we evaluated in-vivo antiarthritic activity of the ethanolic extract of two different formulations containing the 300 mg/kg orally and 600 mg/kg body weight, it was evaluated using different methods such as complete Freund’s adjuvantinduced arthritis, turpentine oil and formaldehyde induced arthritis. Results: The result revealed that the formulations PHF3 and PHF4 possessed significant in-vitro anti-arthritic activity by protein denaturation inhibition and HRBC membrane stabilization comparing with the standard drug diclofenac sodium. In turpentine oil, formaldehyde, and complete Freund’s adjuvant-induced arthritis models, the polyherbal extract formulations significantly (P < 0.001) reduced joint and paw swelling and markedly improved body weight, haematology profile and parameters in complete Freund’s adjuvant model Conclusion: It could be concluded that the above two polyherbal ethanolic extracts are an effective for antiarthritic activity, supporting its traditional use in the treatment of rheumatoid arth

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