Background: There are countless advantages to breastfeeding for the child and the mother. Breastmilk is the physiological standard for baby nourishment, providing mothers and babies with several health advantages and protection. Nursing mothers may confront a slew of obstacles in their quest to fulfill their breastfeeding goals. Exclusive breastfeeding is affected by maternal, infant, and socio-environmental constraints. Aim: The aim was to identify the main barriers to exclusive breastfeeding for nursing moms in rural and urban areas. Methods: The study included 200 Lactating mothers with an infant 0-6 months. Participants were women aged 18–35 years, in Rural and Urban areas of Lucknow City. Self-administered Socio-Demographic Questionnaire and Scale of Perceived Barriers to breastfeeding by Nhan Thi Nguyen, Houng Thi Do, and Nhu Thi Van Pham (2021) were used for data collection. Results: The results showed that women between the ages of 20 to 30 face greater barriers to breastfeeding, whereas urban area women have more barriers to breastfeeding than rural areas. Conclusion: In this study, lactating mothers are aware of exclusive breastfeeding, but more information is needed about breastfeeding to improve breastfeeding practices and reduce the odds of exclusive breastfeeding.