Extraction of phenolic compounds and betalains from plant matrices represent a real challenge to researchers due to the complexity and diversity of bioactive compounds. In this study we used the response surface methodology to address the effect of three important extraction factors; Temperature, time and solvent content on extraction yield from the barbary fig fruit, Opuntiaficusindica. Temperature range was between 0 and 80°c, Time between 0.36 and 96 h and methanol content between 0 % and 100 %. The goal was to identify the impact of each parameter on extraction yield. Our results show that pulp had the highest betalain content and peel exhibited the highest total phenolic compounds. Polyphenols and betalains from pulp are more extractable using methanol solvent and from peel using water. Optimal condition for betalain content of 19.6 mg/100 g was obtained from pulp using 1.37 °C, 100 % methanol for 0.36 h. However, Optimal condition for total phenolic compounds of 2.77 mg GAE g-1 was obtained using 2.38 °C, 0 % methanol content for 96 h.