.

ISSN 2063-5346
For urgent queries please contact : +918130348310

OLIGO- AND POLYSACCHARIDES FROM THE AERIAL PART OF PLANTS EREMURUS FUSCUS

Main Article Content

Turdumambetov Keneshbek1, Azhibaeva Zulaika Sulaimanovna2*, Ernazarova Elnura Esenbaevna3, Kasieva Gulsara Keneshovna4, Orozmatova Gulnur Tynchylykovna5, Bakirova Aida Adilbekovna6, Murzakulova Batma Sydykovna7
» doi: 10.31838/ecb/2023.12.si5.055

Abstract

Background: For the first time, the carbohydrate composition of the above-ground part of E.fuscus plants growing in Kyrgyzstan was studied; fructosans, water-soluble polysaccharides, pectin substances and hemicellulose were isolated. Aim: To establish the type of link between hexoses, glucomannan was methylated according to the Hakomori method. The completeness of methylation was checked by double repetition. 2,3,6-tri-O-Me-D-mannose (67.5%), 2,3,4,6-tetra-O-Me-D-mannose (13.5%) and 2,3-di-O-Me-D-glucose 4.0%. Materials and Methods: The crushed dry aerial parts of plants were treated with ethyl alcohol (96%) at a ratio of 1:8, and heated in a water bath for 1 hour. The extract was filtered, the raw material was extracted twice with 82% ethanol. All alcoholic extracts were combined and evaporated to half the volume, treated with activated carbon at 60-65ºC for 10 minfiltered several times. The clear solution was evaporated at 40-45ºC to a thick syrup. The syrup was treated several times at room temperature in a ratio of 1:8 with concentrated isopropyl alcohol, then acetone, and ether. Oligosaccharides were stored in a vacuum desiccator, which is a slightly yellowish powder, easily spreading in the air. Results: As a result of extraction with ethanol, oligosaccharides, fructosans, were isolated from the aerial part of E. fuscus, and the quantitative ratio of fructose and glucose was determined. A water-soluble polysaccharide isolated from the remains of raw materials, consisting of mannose and glucose, which is glucomannan, is a slightly yellowish powder soluble in water. According to gel filtration, the molecular weight is 33500, the ratio of glucose and mannose is 1:3.5. Conclusion: In the IR spectrum of glucomannan there are absorption bands 815cm-1-hexapyranose ring, 840cm-1-equatorial bending vibration of α-sugars, 1040cm-1- are characteristic of bending vibrations of axial C-H bonds, 1240cm-1-ester bonds, 1635cm-1 - adsorption water.

Article Details