Background: Entrepreneurship has recently risen to the top of the list of crucial considerations in the study of economic growth processes, along with innovation and competitiveness. Aim: This study aimed to assess nursing personnel perception toward entrepreneurship at Beni_Suef University Hospital. Design: A descriptive research design was utilized to conduct this study. Setting: This study was conducted at Beni_Suef University Hospital. Subjects: All available head nurses and charge nurses (80) of a convenience sample at the time of data collection in the fore mentioned setting. Tool of data collection: One tool was used in this study named: Nursing personnel perception toward entrepreneurship questionnaire. Results: Findings of this study showed that, more than two-thirds of the studied subjects had un-satisfactory level of total entrepreneurial knowledge, more than half of the studied subjects had an in-competent level of total entrepreneurial skills, about two-thirds of the studied subjects had a negative attitude regarding entrepreneurial. Conclusion: There was about two thirds of the studied subjects had a un satisfactory perception level regarding entrepreneurship. Recommendation: Conduct workshops and training programs regarding entrepreneurship, particularly in the health care field.