Antifungal drug resistance and a lack of clinical response in 20% of cases of dermatophytosis demanda valuation of resistant dermatophytes using a standardized simple and reproducible in vitro analyze, to permit a clinician to select the suitableantifungal agent based on the susceptibility of the isolate to the antifungalagent. Skin, hair and nail samples were collected from patients already under antifungal treatment cases of dermatophytosis. A total of 118 isolates were tested for minimum inhibitory concentration (MIC) against four antifungal drugs in the study. Itraconazole, ketoconazole, terbinafine and fluconazole, was the antifungal drugs tested using the broth microdilution method. MIC50 and MIC90 values were recorded.A total of 118 dermatophytes isolates were tested. Dermatophytic isolates in this study were T. mentagrophyte-80, T. rubrum- 31, T. interdigitale-05 and M. gypseum-02. MIC90 value for terbinafine and fluconazole was significantly higher for all isolated dermatophytes. MIC of 68.64 % isolates for terbinafine and 35.59% isolates for fluconazole were lower than the cut-off value, which indicates that not all treatment failure cases are due to drug resistance.