The aim of this research was to create a green synthesis of metallic nanoparticles using leaves extracts and isolated compounds of Acacia catechu (L.) Willd and Acacia auriculiformis A.Cunn. Extract and isolated compounds along with excipents were used to prepare Silver nanoparticles and was evaluated for particle size, zeta potential and % EE. The synthesised AgNPs colloidal solution shown superior antibacterial activity against both Gram-positive and Gram-negative bacterial strains i.e., Klebsiella pneumonia, Bacillus subtilis, E. coli and Streptococcus sp. in testing. The diameters of the inhibition zones of AgNPs at 50 g/ml concentrations against Klebsiella pneumonia, Bacillus subtilis, E. coli and Streptococcus sp. were reported and it was found that the Silver Nanoparticles have significant antibacterial activity