Lack of knowledge regarding polycystic ovary syndrome and poor lifestyle is considered to be the major factor leading to polycystic ovary syndrome. Aim: To evaluate lifestyle and reproductive health in female nursing students about polycystic ovary syndrome: teaching program. Study design: A Quasi- experimental design was utilized in the present study. Setting: The study was carried at Faquos nursing technical institute the study was carried at Faquos Nursing Technical Institute from the first of November 2022 to the end of March 2023. Subjects: A Convenient sample was used in the study (200 females nursing students). Tools: A structured interview tool, lifestyle assessment tool and reproductive health knowledge assessment tool were used for data collection. Results: There was a marked improvement in all subscales of students’ lifestyle and knowledge post implementation of educational program with a highly statistically significant difference at (P= < 0.001). There was high a significant statistical positive correlation between female nursing students’ lifestyle and knowledge during pre (r=0.514), post (r=0.641) and follow up phase (r=0.688) of educational program. Conclusions: The educational program significantly improved the lifestyle for students concerning diet, physical activity, body mass index, leisure time, sleeping and emotional status. Educational program was effective in improving the knowledge of female nursing students regarding PCOS. Recommendations: Implementation of an educational program about main items of PCOS and negative effects on the reproductive health for all students (medical and non-medical).