Cardiospermum Halicacabum is known as a medicinal herb, and its seed is used in herbal medicine. Lipases are found in a variety of taxa including bacteria, mammals and plants. However, research has shown that plant lipases are involved in numerous physiological processes other than triacylglycerol hydrolysis. The aim of this research was to get a deeper insight into the characteristics of the lipase activity in germinating C. halicacabum seeds the purified enzyme was quantified by HPLC as well SDS-PAGE analysis to perform a more detailed analysis at different pH, Temperatures, of their Lipase activity was done using coconut oil as substrate.