Efficient biodegradation of cellulosic biomass needs a battery of enzymes targeting cellulose, hemicellulose, and lignin. Termites cause damage to both cellulose and non-cellulose containing substances like living trees, crop plants, due to its symbiotic association with different microorganisms. Hence in this study, gut microflora of termites with cellulolytic potential were screened and identified. Termites were collected from different locations of Mandsaur and Neemuch Districts of Madhya Pradesh Malwa region. The microflora of termites gut were isolated and cultured on CMC media for Cellulolytic strains identification. Total 56 bacteria were isolated from the gut of Termites, whereas 31 were found with cellulolytic activity through screening on carboxymethylcellulose (CMC) media. Among 31 cellulolytic active bacteria, five bacterial isolates showed more promising cellulolytic activity than others. The five cellulolytic strains were further analysed for the accurate molecular identification through the PCR amplification of 16S rRNA gene followed by Sanger sequencing. Based on 16s rRNA gene sequencing, we have identified Cellulolytic bacterial strain as Bacillus sp. (in: firmicutes) strain NTG 4, Aneurinibacillus migulanus strain MTG 15, Bacillus subtilis strain STG 8, Streptomyces pseudovenezuelae MTG 17, Bacillus megaterium strain MTG 19. The B. megaterium strain MTG 19 showed highest cellulolytic activity followed by Bacillus subtilis strain STG 8 in these five cellulolytic isolates.