Biosurfactants are the sustainable options for ecologically friendly remediation of hydrocarbon contaminations. The study aimed at isolation and characterization of biosurfactant producing isolates from hydrocarbon contaminated soil from Satara district in Maharashtra, India. Drop collapsing and oil spreading tests and CTAB tests of fermented media were primary screening tests for screening putative isolates. Blood agar lysis, lipase, surface tension reduction and emulsification measurements were used as quantitative tests. Three potential bacterial isolates with significant biosurfactant activities were found. The biochemical characterization and 16S rRNA gene classified the isolates as Achromobacter xylosoxidans, Bacillus subtilis Pseudomonas aeruginosa and were registered in GenBank with the accession number OP060997.1, OP002276.1 and OP846851.1 respectively