Charak samhita has described the role of urine in anointing, pasting, enema, purgatives, fomentation and abdominal distension. Urine is used in poisoning also. Urine endowed with properties of being sharp, pungent, saline and non unctuous is useful in diseases like piles, skin disorders, white patches on skin, abdominal diseases. Urine promotes appetites and digestion. Urine acts as anti poison and kills worms in the body. Urine gives appreciable results in anemia. Ayurvedic texts have described properties of eight types of urine. Urine is used in the form of internal application by drinking and through its external application by mixing it with some powdered drugs. The objective of present work was to that horse urine an ancient medicine (Charak samhita) used for treatment of pain by tribal of India. Present proposal for research for Isolation and characterization of active constituent from Ashva urine for treatment of pain and made an effort to provide scientific claim of Ashva urine.