Docetaxel (DTX) and dutasteride (DTE) nanostructured lipid carrier (D/D-NLC) was constructed to exert anticancer effects against Prostate cancer cell lines.(D/D-NLC) were prepared using the Melt-emulsification technique and high pressure homogenization. D/D-NLC showed homogeneous particle size (< 206.4 nm) with zeta potential varying from -23.3 to -38.6 mV. DTX DTE was successfully encapsulated in the NLCs: encapsulation efficiency (> 98%); drug loading (8 − 18%). All NLC formulations were stable for 5 weeks under the storage conditions at 4 °C. D/D-NLC loaded formulations assayed cytotoxicity by MTT assay. The assay measures the cell proliferation rate and conversely when metabolic events leads to apoptosis or necrosis, there was the reduction in cell viability.