In a country like India the majority vehicles are running on ICE for which propulsion is derived from petro diesel products. Over the years it is observed that petro diesel resources are depleting in nature. On the other hand, the pollution levels are increased tremendously. To overcome these problems researcher have concentrated on alternative fuels in place of petro diesel fuels. The biodiesel properties are closer to diesel. In this work palasa seed oil is used as substitute fuel with various blend combination. Palasa seed oil is combined with regular diesel in the amounts of B10, B20, B30, and B40. The research is being conducted on a direct injection single cylinder four stroke diesel engine. Among the evaluated blends, B20 has the closest performance and emission characteristics to diesel. When compared to B0 blend, the brake thermal efficiency of B20 mix is enhanced by up to 13.41%. Further the HC, CO emissions are reduced with B20 compared to standard diesel. However, the oxides of nitrogen emission levels are slightly increased compared to B0 blend.