Micropropagation is a method to grow plants in a sterile environment from tissue culture. The objective of this study was to investigate the feasibility of micropropagation of mint (Mentha spp.) and evaluate the antibacterial activity of mint extracts. The in vitro regeneration of mint varieties was performed on Murashige and Skoog (MS) medium supplemented with various plant growth regulators. The established in vitro propagated plantlets were subjected to acclimatization, and the plantlets were transferred to greenhouse conditions. The antibacterial activity of the methanolic extract of mint was evaluated against Gram-positive Bacillus cereus and Gram-negative Escherichia coli using the Disc diffusion method. Mint extracts exhibited a considerable inhibitory effect against both the tested bacteria.