Introduction: Rheumatoid arthritis is a globally distributed systemic autoimmune disorder which mainly affects joints. It has a higher prevalence in the industrial area due to environmental risk factors or genetic factors. An encouraging source of novel chemicals with strong anti-inflammatory properties may be found in the enormous diversity of the plant kingdom. Natural remedies have made a tremendous contribution to the advancement of modern medicine. Recent worldwide reviews of conventional medicine have been influenced by extensive study on numerous plant species and their active medicinal components. The aim of the current study was to compare and assess in-vitro anti-arthritic effects of four solvent (petroleum ether, ethylacetate, methanol, water) extracts of three plants Commiphoramukul, Nyctanthusarbortristis, Eucalyptus globulus against the denaturation of protein inhibition assay in-vitro. Methods:In order to evaluate the anti-arthritic property, the test extracts at various concentrations were incubated with egg albumin under highly regulated laboratory conditions and then evaluated for absorbance to determine the anti-arthritic activity. Results: The percentage inhibition of protein denaturation was calculated. The current findings showed that all test extracts inhibited protein (albumin) denaturation in a concentration-dependent manner. Conclusion: It was determined that the methanolic extract of Commiphoramukul was the most effective of all extracts. Further, all of the extracts have a significant anti-arthritic impact against in-vitro protein denaturation inhibitionassay.