The use of medicinal plants to treat diseases is the most common remedy. The seeds of Alangium salvifolium are rich in phytochemical components. They exert antioxidant and anticancer effects known for their beneficial effects on human health. The aim of this study was to investigate the antioxidant and cytotoxic potential of Alangium salvifolium seed extracts against human skin melanoma (A375) and human skin carcinoma (A431) cell lines in vitro. Phytochemicals and antioxidant activity were investigated in ethanol and ethyl acetate extracts of Alangium salvifolium seeds using standard methods. In vitro cytotoxicity assays were performed against human skin melanoma (A375) and human skin carcinoma (A431) cell lines using the MTT assay. There was results showed that Alangium salvifolium has antioxidant potential. The observations of the MTT cell cytotoxicity study indicate that the ethanolic and ethyl acetate extract of the plant exhibits significant cytotoxic properties against the A431 cell lines and moderate cytotoxic properties against the A375 cell lines. Overall, the ethanolic extract shows effective antioxidant potential and cytotoxicity against both A431 and A375 cell lines. It appears that Alangium salvifolium seed extracts may prove to be promising anti-cancer cell agents if the ethanolic extract is specific. However, further studies are needed to confirm these results in vivo.