In India, numerous traditional practitioners employ the evergreen tree Trigonella foenumgraecum to heal gastric ulcers. The goal of the current study was to assess the in-vitro antiulcer activity of Trigonella foenum-graecumseed aqueous extract (AE). In this study, we performed anti-ulcer activities using aqueous extract concentrations of 100 mg, 500 mg, 1000 mg, and 1500 mg in an in-vitro method for the H+/K+ - ATPase inhibition activity method. In the acidneutralizing capacity (ANC), the extract significantly decreased ANC as compared with standard Aluminium hydroxide + Magnesium hydroxide (500 mg). Significant antiulcer action has been found in Trigonella foenum-graecumseed powder, and this species' active ingredients have been thoroughly studied.