Chlorophytum tuberosum, a plant species notable for its significant medicinal properties, has seen a worrying decline in its natural populations due to increased commercial and medicinal exploitation. This study investigates the potential of in-vitro callus culture as a sustainable propagation method for this species, specifically aiming to identify optimal growth conditions and analyze the bioactive compounds produced during development. The plant materials used were collected from Sangamner Tehsil and subjected to surface sterilization