Infertility is frequently a silent struggle for couples. Factors of both female and male are equally accountable, thus it has become a global concern. Male infertility is mainly due to the suboptimal quality and quantity of sperm. Seminal components play an important role in understanding male infertility. Plasma components of seminal “(SP)” play a vital role in metabolism of sperm, along with function of sperm, their survival, and transportation in the reproductive tract of female. “(SP)” is known to contain broad variety of macro- and microelements. The human seminal fluid is rich in elements like calcium, zinc, magnesium, iron, and other trace elements. With these attributes, a thorough examination of SP may serve as a good predictive marker to evaluate male fertility and reproductive disorders in addition to the physical characteristics of semen. This review paper focuses on the properties of biochemical with current understanding of human seminal plasma along with their relevance to ascertain male fertility.