In order to assess the effects of pesticide use as an agricultural practice on soil health, soil samples were collected from three different eco-environments (forest, agriculture, and urban), and the comparative nutritional status was examined. A survey was also conducted with 65 farmers from 8 villages in Dehradun (Uttarakhand) to assess the effects of pesticide use on farmers' health as well as to assess farmers' knowledge and practices surrounding pesticide use. Pre-tested questionnaires were used to gather data on the aforementioned criteria, which were then, analyzed using the right statistical software. 93% of respondents used pesticide, 44.6% were aware of its effects on health and the environment, 64% disposed of leftover pesticide in the field, and 18% reported having complaints about various health conditions. Significant correlation was found between health problems and disposal methods. Regarding pH and conductivity, the nutritional condition of soil from three ecosystems varied greatly. Forest and agricultural soil samples had considerably different levels of organic carbon, potash, and iron. The amounts of copper in soil samples from agricultural and urban areas varied significantly.