The synthesis of new materials with decent luminescent properties signifies a challenging but imperative contribution due to their potential applications in biomedical science. In this study we evaluate the potential combinations of nanohydroxyapatite and brucine for various biomedical applications. The main objective of this study was to revel the influence of brucine ions on the design and structure of hydroxyapatite. Different concentrations of brucine (0.5%, 1%, 2%) has been incorporated with nanohydroxyapatite. The morphology and structure, as well as the optical properties, of the obtained nanomaterials were characterized using X-ray powder diffraction analysis (XRD), Fourier Transform Infrared spectrometry (FTIR), SEM microscopy, UV-Vis spectroscopy. The measurements revealed that brucine ions were integrated into the structure of hydroxyapatite in all the composition. The biocompatibility and cytotoxicity of the obtained powders evaluated using MTT assay and fluorescent spectroscopy reveals the ability of the synthesized nanomaterials to be used for biological system imaging.