Authors are grateful to the Deanship of Research-Bethlehem University for financial support through the Palestinian Ministry of Higher Education and Research. Abstract: In this study, thirty samples of skin-whitening creams were analyzed for total hydroquinone by High Performance Liquid Chromatography. The samples were divided into four groups: group (1) samples from Raed Cosmetics Factory (local product), group (2) samples from different pharmacies in Bethlehem, group (3) products form black market and group (4) handmade product. The concentration of hydroquinone ranged from below 0.001% to 5.30%. It was realized that three percent of cream samples analyzed were contained amount of hydroquinone higher than the recommended World Health Organization (WHO) and Food and Drug Administration (FDA) maximum permissible limit of 2%.z