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ISSN 2063-5346
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HPLC ANALYSIS OF PHENOLIC COMPOUNDS FROM ARTEMISIA SPECIES

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Bianca Ivanescu,[a]* Laurian Vlase,[b] Cristina Lungu[a] and Andreia Corciova
» doi: 10.17628/ECB.2016.5.119

Abstract

The aim of this study was the identification and quantification of biologically active compounds from three Artemisia species, used as remedies in Romanian traditional medicine. A new LC-MS method was developed for the analysis of methoxylated flavones and also the quantification of caffeic and chlorogenic acids was performed. The antioxidant activity of plant extracts was evaluated by DPPH radical scavenging assay. We report for the first time the presence of eupatorin and hispidulin in A. absinthium and A. vulgaris, of eupatilin in A. annua and casticin in A. vulgaris. Jaceosidin and acacetin were not identified in any sample. Although all extracts showed antiradical scavenging activity, A. vulgaris exhibited the strongest antioxidant effect. Chlorogenic acid was found in high amounts in all species, notably in A. annua and A. vulgaris.

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