Introduction: Obesity is a crucial component of metabolic syndrome, which also includes dysglycemia, high blood pressure, elevated triglyceride levels, and low levels of HDL cholesterol. A metabolic syndrome is a group of interconnected risk factors for diabetes and cardiovascular disease. Hyperlipidemia caused by a highfat diet is one of the most prominent risk factors for atherosclerosis and coronary heart disease around the world. For more than 20 years, statins have been the most routinely prescribed drugs for the treatment of hyperlipidemia Aim: The aim of the study is to elucidate the Histopathological and histochemical analysis of heart muscle and aorta of Wistar rats exposed to a high-fat diet Materials and method: A total of 4 formalin fixed paraffin embedded tissue blocks of Wistar rat samples that were exposed to high-fat diet and 2 control rat samples. Four lysine-coated slides were obtained from each block and used for further processing. Results: The expression of Alpha SMA is significant in High-fat diet samples compared to control samples. The expression of S100 is increased compared to control samples. Increased fibrosis was seen. Conclusion: Overall, the findings obtained herein suggest exposure to HFD can alter cardiac integrity and induce heart fibrosis.