In current years, the sustainable approach of synthesizing silver nanoparticles (AgNPs) has gained much interest amongresearchers. The flora of the Indian field is rich with a variety of medicinal plants thathavethe potential to include various sources of effective, cost-efficient, non-toxic, environmentally safe reducing and stabilizing compounds that can be utilized in synthesizing in AgNPs. Here, in this study, the author investigates an efficient and sustainable route of AgNP preparation from 1 mM aqueous AgNO3 using leaf extracts of Tribulusterrestris well enriched for their vast availability and therapeutic property against Urinary tract infections (UTIs) samples.