Biogenic synthesis of silver nanoparticles has attracted researcher’s attention in recent days because of the necessity to develop new clean, cost effective and efficient techniques. In our study, we report green biogenic approach to synthesize silver nanoparticles using flower extract of Rosa damascena as reducing and stabilizing agent. The phytochemicals present in the petal extract induced the reduction of Ag+ ions which resulted in the formation of conjugated nanoparticles. The formation and stability of the as-produced nanoparticles were monitored by UV-visible spectrophotometer that demonstrated an absorbance peak at around 412 nm corresponding to the plasmon resonance of silver nanoparticles. The average crystallite size of the particles calculated from the obtained XRD spectra using Debye Scherrer’s formula was 4 nm. TEM results confirmed the formation of nano-sized polydispersed quasi-spherical shaped particles having the average diameter size of approximately 20 nm. Analysis of FTIR spectra revealed that the chemical moieties containing mainly amine, carboxyl, alcohol, esters functional groups coated the surface of the nanoparticles. Obtained nanoparticles were highly stable with no agglomeration even it is preserved for a period of 3 months and showed ‘good’ antibacterial activity.