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ISSN 2063-5346
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GREEN SYNTHESIS OF SILVER NANOPARTICLES USING DENDROPHTHOE FALCATA (L.F) ETTINGSH STEM EXTRACT- AN EPIPHYTIC PLANT: CHARACTERIZATION, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY

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Gnana Priyanka Beulah G 1 , Tresina P. S2 , Mohan V. R3*
» doi: - 10.48047/ecb/2023.12.si5a.0187

Abstract

A phytoextract mediated blend of silver nanoparticles using Dendrophthoe falcatastem extract as capping and stabilizing agent without using hazardous toxic chemicals via green route has been calculated. The green synthesized nanoparticles were characterized by Scanning electron microscopy (SEM), UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Atomicforce microscopy (AFM) analysis. By UV-Vis spectra at 484nm, AgNPs formation was checked. FTIR had shown that the phytochemicals were responsible for the reduction and capping material of silver nanoparticles. The size and shape of the AgNPs were regulated by making use of SEM. The XRD results divulged a crystalline nature of AgNPs with ausual size of 33.56nm. The surface topography was supported by AFM techniques. The antioxidant activity of the plant stem extract and formulated AgNPs have been performed by means of 2- 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay employing ascorbic acid as a standard. The antibacterial activity was regulated by disc diffusion method. This method is used to measure the efficiency of both plant stem extract and its mediated silver nanoparticles against six gram positive bacterial strains like Bacillus thuringiensis, B. subtilis, Streptococcus faecalis, S. pyrogens, Staphylococcus aureus, Enterococcus faecalis and six gram negative bacterial strains such as Salmonella paratyphi, S.paratyphi-A, S.paratyphi-B, Proteus vulgaris, P. mirabilis and Escherichia coli. The antibiotic tetracyclineis used as reference standardfor both bacterial strains. The results illustrate that the Dendrophthoe falcata stem mediated AgNPs may be employed in many biomedical applications and the production of many antibacterial products in the future.

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