Due to plant distinct qualities and many surface capabilities for diagnosis and targeted cell therapies, gold nanoparticles (AuNPs) have been widely used in medical applications. Current nanotechnology research has a fresh focus on environmentally safe green production of metallic nanoparticles using renewable bioresources. Aqueous extracts of Bay leaf, Cassia bark, and Cardamom were used in the current study to create gold nanoparticles (AuNPs) with outstanding stability in a cost-effective and environmentally friendly manner. Spectroscopic analysis was used to assess how reaction variables like temperature and pH affected the biogenesis of nanoparticles. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction assay (MTT Assay) was used to determine the in vitro cytotoxicity of these gold nanoparticles.