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Green Synthesis of Amla Fruit mediated Silver Nanoparticles And its Antibacterial Efficacy against Clinical Pathogens - an Invitro Study

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Dr. Santo Grace Umesh*1, Dr. Rajeshkumar S2, Tharani Munuswamy3
» doi: : 10.48047/ecb/2023.12.12.170


Background: The overwhelming growing need to generate ecologically benign material syntheses represents a bridge between biotechnology and nanotechnology. This has gained increased attention over the past decade. Many different synthetic approaches are being used to find acceptable biomaterials for the production of nanoparticles. India's indigenous system of healing shows that Emblica officinalis holds a sacred place. Formulation of amla- mediated nanoparticle delivery system could serve an extremely evident use in current medical field. Aim:The present study aimed to formulate an amla mediated silver nano particles by green synthesis and evaluate its antimicrobial effects. Materials and Methods:Green synthesis of amla mediated silver nano particles was done by adding appropriate amounts of plant extracts and silver nitrate solutions. They were placed on an orbital shaker at 100rpm and was further centrifuged at 7000rpm for 10minutes to obtain nanoparticles. Antibacterial activity was assessed on three clinical pathogens namely, Streptococcus mutans, Vibrio parahemolyticus and Vibrio harveyi. The bacteria were cultured and two concentrations of the extract and a control (erythromycin) was introduced by disc diffusion method. The zone of inhibition was measured. Results:The change in colour from green to brown indicated the synthesis of nano-particles. It was found that erythromycin had the maximum activity again Vibrio harveyi while 10 μl of amla mediated silver nano-particles had the maximum antimicrobial activity against Vibriyoparahemolyticus and Streptococcus mutans with a mean and standard deviation of 13.66 ± 0.47 and 8.66 ± 0.47 respectively.

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