In this study, a comparitive phytochemical investigation for the antioxidant, antidiabetic, antimicrobial and antifungal potentials of Pearl millet and Kodo millet were performed. Methanolic extract of grains showed high antioxidant and antidiabetic activities. GC-MS analysis of methanolc extracts of Pearl millet and Kodo millet revealed the presence of many bioactive compounds with various therapeutic uses and mass spectra of the compounds obtained were compared with the National Institute of Standards and Technology (NIST) library. In-silico docking studies of the identified compounds in GC-MS was performed to validate the activity of the compounds against active site of alpha glucosidase and alpha amylase. The results of this current study revealed that kodo millet exhibited high antioxidant and antidiabetic activity and can be used as a good source of nutrition with additional health benefits.