Background: The leading cause of infant death and disability, particularly in nations with poor or medium incomes, is neonatal sepsis. The management of newborn sepsis relies on constant surveillance of infections and the development of drug resistance. The purpose of this research was to identify bacterial causes of newborn sepsis, trends in antibiotic resistance, risk factors, and outcomes for patients. Methods: This cross-sectional study was conducted in different hospitals of Peshawar, Kuwait Teaching Hospital, Khyber Teaching Hospital (KTH), Hayatabad Medical Complex (HMC) Lady Reading Hospital (LRH). Total 118 neonates were presented in this study. Approximately 2 milliliters of blood were collected in an aseptic manner and then added to the patient's Tryptone Soya Broth while they rested. We used normal microbiological procedures to identify the bacteria. To find out how each bacterium was sensitive to antibiotics, we employed the disc diffusion technique. SPSS 22.0 was used to analyze all data. Results: There were 67 (56.8%) males and 51 (43.2%) females among all neonates. Frequency of preterm births were 31 (26.3%). Mean weight of the neonates was 3.11±5.13 kg. Most common cause of neonatal sepsis was Klebsiella sp, E. coli and Coagulase negative Staphylococci. The drugs that worked best against both gram-negative and gram-positive bacteria were ciprofloxacin and amikacin. A staggering 82% of the bacterial isolates tested showed signs of multidrug resistance. Conclusion: Among the microorganisms that were often identified in our investigation were Klebsiella spp. and E. coli. Quite a few of the patients showed signs of being resistant to more than one treatment. The need for ongoing assessment of antibiotic resistance rate is highlighted by the fact that the majority of the bacteria that were identified were resistant to ampicillin, ceftazidime, cefotaxime, and gentamycin.