As oral route is the preferred route of administration of majority of drugs but it has certain limitations such as pre-systemic metabolism or first pass metabolism in liver, g.i.t upset and destructions of enzymes. Pregabalin is another synthetic atom and an underlying structure of the inhibitory synapse γ-aminobutyric corrosive. This research was based on the synthesis and in-vitro characterization of mucoadhesive patches of Pregabalin by incorporating different polymers using solvent casting method. Pregabalin (API) was obtained as gift sample from the Dr. Reddy’s Pvt Ltd Hyderabad. The Ethanol, Eudragit L-100, Propylene glycol, Tween 80, Methanol and HPMCK4M & HPMCK15M and hydroxypropyl methylcellulose (HPMC) were purchased from Sigma Aldrich Pvt Ltd India. The formulation was evaluated for parameters like weight variation& thickness, flatness, folding strength, moisture content, drug content determination, diffusion cell in-vitro model, surface pH, Water Vapor Transmission rate determination, In-Vivo patch test, Pharmacological screening of formulated patch in Neuropathic pain and Stability studies. In results, an excellent in-vitro release rate of the formulation was demonstrated by the buccal patch. In order to estimate stability profile of mucoadhesive patches, it was evaluated for physical appearance. After 30 days of storage, physical appearance for different patches was found same. Pregabalin incorporated in the mucoadhesive patches may be frequently employed in the modulation of numerous CNS activities with better absorption. It concluded, a better in-vitro scattering time alongside rich appearance and other actual attributes like elasticity, % lengthening, collapsing perseverance. P2 was chosen dependent on its outcomes on performed assessment which were all ideal and furthermore had great mechanical properties. It would be very beneficial for the management of neuropathic pain as stable mucoadhesive patches consisting pregabalin.