The stiripentol nanosuspension formulation was developed via emulsification solvent evaporation employing Soluplus, Vitamin E, Sodium Lauryl Sulphate, Poly Vinyl Alcohol, Methanol, and adequate water as excipients. Formulations SF1-SF16 entrapped 80.28±1.28% to 98.46±1.67%. The Zeta potential for optimized Formulation SF4 was -4.49 mv, which was acceptable. The Optimized SF4 nanosuspension particle size was 110.4 nm. From invitro testing, Formulation SF4 released the most drug in 60 minutes at 99.49±1.43, but the other formulations did not. Zero-order, first-order, and equation models mentioned Nanosuspension drug release. Based on regression values, Formulation SF4 optimized follows first-order kinetics.