Objective: The aim of present study was to formulate sustain tablet of anti-diabetic drugs Nateglinide and Vildagliptin to improve prolongation of action and reduce dose frequency. Nateglinide and Vildagliptin have short half-life of 1.4 hours and 2-4 hours respectively so they rapidly absorbed after oral administration and upper part of intestine. The objective of the research work was to retain both the drugs for prolonged period of time. Method: The sustained release tablets were prepared with polymers like xanthan gum and HPMC K100 M as release retarding polymers prepared by wet granulation method. The prepared sustain release tablets were optimized by Central Composite Design by using Design Expert software trial version. Precompression parameters and post compression parameters like, hardness, friability, weight variation, drug content and In Vitro dissolution drug release were performed. Result: The results of all parameters were within acceptable limit. There was a significant difference in in vitro drug release because of xanthan gum and HPMC K100 M and its concentration. The release of Nateglinide and Vildagliptin was found to be 99.03±3.48 and 98.99±2.10 respectively in 24 hours. The release mechanism followed Higuchi release kinetic. Optimization was done by using Central Composite design. There were no any significant changes after stability study of NV9 batch. Conclusion: The development of Sustain release tablet was attempted and present study indicate that NV9 batch shows sustain effect of Nateglinide and Vildagliptin for 24