AIM: The purpose of this study is to construct and examine an in-situ gel using a pH-triggered method and a mixture of prednisolone and moxifloxacin. The ocular has a low bioavailability and a constrained efficacy in eye drops because of lacrimal fluids. The effectiveness of the in-situ gel-forming mechanism has diminished in the eyes and changed from sol to gel in the cul-de-sac. Materials and procedures: The crosslinked polyacrylic acid polymer in the pH-triggered in-situ gel containing Carbopol 940 has a broad-spectrum mechanism based on the addition of pathogens. The mucoadhesive polymer hydroxypropyl methylcellulose (HPMC) is used to increase viscosity. The pH was changed to 6.5 by using buffering agents in the proper amounts. Clarity, pH, gelation temperature, spreadability, and medication concentration were all assessed for each formulation. CONCLUSION: The pH-triggered in-situ gel was created, and evaluations of its transparency, gelation, and pH found them to be adequate. The continuous release of medication over long lengths of time made possible by this formulation of pH-triggered in-situ gel systems makes it more stable, non-irritating, and medically efficacious than conventional eye drops.