Polymeric microspheres are one of the novel drug delivery system. Canagloflozin is a first oral agent in novel class of diabetes drug as SGLT2 inhibitor. Sodium glucose co-transporter2 (SGLT2) inhibitor will reduce the risk of a first hospitalization for heart failure, possibly through glucose-independent mechanisms. The inhibition of SGLT2 limits renal glucose reabsorption, promoting its urinary excretion and the reduction of plasma glucose levels. Thereby, SGLT2 inhibitors use a novel mechanism of action, since they do not interfere with insulin secretion. The main objective of the work is to prepare canagliflozin loaded microspheres for controlled release and for anti-microbial property by using natural polymers Chitosan and Sodium alginate. Formulation was prepared by Solvent Evaporation method with implementation of full factorial design, varying the drug-polymer ratio and stirring speed. The 32 factorial design was implemented means twofactors and three-levels are fitted in a statistical model to evaluate the responses. The microspheres were evaluated for micromeritic properties, particle size, production yield, swelling index, entrapment efficiency, t50, drug polymer compatibility (DSC and IR Studies), in-vitro drug release, kinetic models of microspheres. The percentage drug release 98.85% for a period of 15 hours. The result shows that as the concentration of polymer increases it affects the particle size, percentage yield, and in vitro drug release of microspheres. As the drugpolymer ratio increases production yield will also be increased, with increasing the stirring speed the particle size will be decreased