Objective: The primary target of this study was to formulate and invitro study of BSA loaded nanocrystal containing paclitaxel as a novel method by desolvation technique. The impact of various test on the particle size, entrapment efficiency, percent drug released etc. was assessed. The 32 full factorial designs was employed to investigate the influence of formulation variables on nanocrystals characteristics. Method: BSA loaded nanocrystals were prepared by using desolvation technique. Nanocrystals were prepared by using BSA and PVP K 30; these were characterized for various methods such as shape, size and, entrapment efficiency and Zeta Potential, Drug excipients compatibility which is determined by FTIR. Result: FTIR shows there was no interaction between formulation ingredients. The average particle size was found251.3 nm. The size of nanoparticles increases with BSA-PVP polymer concentration. Prepared nanocrystal shows drug content 96.04% and Entrapment Efficiency 92.05% with particle size 251.3 nm. In-vitro drug releases shows maximum 96.07%. Conclusion: By using desolvation method BSA loaded nanocrystals was successfully prepared and evaluated, containing good particle size, EE% and zeta potential % drug release so by doing further in vivo study it could be good choice for conventional drug delivery system.