Glibenclamide (GBC) is an oral anti hyperglycaemic agent having low bioavailability and it also produces remarkable hypoglycaemia and frequent Gastro Intestinal (GI) side effects. In the present study, Glibenclamide loaded transfersomes were developed for transdermal delivery by modified film hydration method to improve its bioavailability and evaluated for their vesicle size, shape, PDI, Zeta potential, entrapment efficiency, % drug content and ex vivo skin permeation study using rat abdominal skin. The optimized formulation F2G consist of Lecithin and Tween 80 at a (lipid: surfactant) ratio of 9:1 showed high entrapment efficiency (98.4%) and vesicle size of 168.9 nm with a Zeta potential of -23.2mV and PDI (0.289). The skin permeation studies of F2G showed that the maximum % drug release (81.2%) and high flux rate of 29.6μg/cm2/h. The flux was showed significantly higher (4.35 times) than the drug solution.