Purpose: The present work deals with the formulation and optimization of pectin-based colon specific microsphere formulation containing Resveratrol followed by comprehensive in vitro and in vivo evaluations thereof. Methods: Pectin based microparticles containing Resveratrol were prepared and optimised using Box-Behnken design and coated with Cellulose acetate phthalate. The formulations were evaluated by in vitro dissolution test in simulated gastric, intestinal, and colonic fluid with and without 4% w/v rat caecal matter. Drug-excipients compatibility study was also performed by FTIR spectrometric analysis. Drug release pattern was evaluated by mathematical modelling. In vivo pharmacokinetic study was also performed and the optimised formulation was evaluated for efficacy in a TNBS rat model of ulcerative colitis in vivo