This study's goal was to investigate possible anti-TB and anti-diabetic properties of various extracts of Rhynchosia heynei, a plant traditionally used in Ayurveda and Unani medicine. Preliminary phytochemical analysis was performed to determine the composition of the extracts. In vitro assays were carried out to evaluate the potential anti-diabetic activity by measuring glucose uptake and alpha-amylase inhibition, as well as anti-TB activity by determining the minimum inhibitory concentration (MIC) against Mycobacterium kansasii strain.In comparison to the control group, the findings demonstrated that all the extracts increased glucose uptake in a dose-related manner. Furthermore, the aqueous extract demonstrated alpha-amylase inhibition at 617.6 μg/ml, while the ethanol and ether dilutions had IC50 values of 103.9 and 135.1 μg/ml, respectively, indicating potential anti-diabetic activity. The study's anti-TB findings revealed that the hexane extract had the lowest IC50 (1.01 μg/ml) followed by the ethanol extract (7.599 μg/ml), ether extract (32.7 μg/ml), and aqueous extract (793.3 μg/ml). The presence of phytochemical compounds such as alkaloids, tannins, saponins, steroids, flavonoids,terpenoids, and phenolic compounds in the extracts of Rhynchosia heynei may explain the observed pharmacological activities of the plant. In conclusion, these extracts have the potential to be developed as natural anti-diabetic and anti-TB agents.