Chemical and microbiological contamination of ground water has several implications for human health. The study of chemical and microbiological contamination of water is carried out in Sitnica river (Kosova). Water samples are collected at 10 sampling points and fourteen different parameters are investigated. The program, Statistica 6.0, is used for calculations of basic statistical parameters and anomalies (extremes and outliers). The chemometric approach and the results yielded useful information about water quality and can lead to better water resource management. Dendograms and box-whisker plots are drawn to evaluate the chemical variation. The level of some microbiological and phsico-chemical parameters and eco-toxic ions from surface water are compared with the results from the river source where anthropogenic effects are negative. Our results show that chemical and microbiological pollutions are the results of anthropogenic factors coming from settlements, peripheral rivers and agricultural in the region surround.