Background: Nutrition helps the damaged liver to function properly, reduces the risk of infection and The study aims to the possibility of dependence some physiological criteria (renin, angiotensinconverting enzyme, and nitric oxide) as indicators for early diagnosis of diabetic nephropathy in patients with type 2 diabetes and avoiding its development to advanced stages. The study included the followup of (90) men, (30) healthy individuals and (60) patients with type 2 diabetes to periodic clinical examination, and evaluation of physiological parameters represented in the estimation of glycosylated hemoglobin ratio (HbA1c), levels of renin, angiotensin-converting enzyme, nitric oxide, and albumin to creatinine ratio(ACR) .The results showed a significant increase (P< 0.05) in the glycosylated hemoglobin ratio, the levels of renin, angiotensin-converting enzyme (ACE), monocyte chemoattractant protein and the albumin to creatinine ratio in urine (ACR), and in contrary to a significant decrease (P < 0.05) in the level of nitric oxide. The trial also diagnosed a notable effect (P < 0.05) for the age groups on the glycosylated hemoglobin ratio and albumin to creatinine ratio (ACR),while no noticeable differences appeared (P > 0.05)in levels of renin, angiotensin-converting enzyme ,and nitric oxide, also a significant impact (P < 0.05) was observed for the duration of diabetes mellitus on the levels of renin, and the albumin to creatinine ratio in the urine ,whereas no significant differences revealed (P > 0.05)in levels of angiotensin-converting enzyme and nitric oxide. In addition to the foregoing, the study recorded a significant effect (P < 0.05) of body mass index on the levels of renin, angiotensin-converting enzyme, and the albumin to creatinine ratio in the urine, in contrast no observable differences appeared (P > 0.05)in nitric oxide and glycosylated hemoglobin ratio. Moreover to the significant effect (P < 0.05) for stages of diabetic nephropathy on glycosylated hemoglobin rate, renin levels, ACE, nitric oxide and the albumin to creatinine ratio (ACR) in urine. It was concluded from the study that increased levels of renin and angiotensin-converting enzyme, and unlike it, decreased levels of nitric oxide is considered important predictive vital markers for progression the diabetic nephropathy to advance stages.