Objectives: To evaluate the drug release profile and degradation potential of carrageenan based modified triple antibiotic hydrogel at a concentration of 1 mg/ml. Materials and Methodology: Using the ultraviolet (UV) spectrophotometric approach, the amount of antibiotic ( ie, amoxicillin, metronidazole and ciprofloxacin) released from the hydrogel over a 7-day period was calculated.The assessment of drug release was continued for 7 days at a wavelengths specific to each antibiotic.At various times and with various pH levels, the hydrogel's ability to swell was investigated. 100 mL of distilled water were used to submerge the hydrogel.After removing the extra surface water with tissue paper, the weight of expanded hydrogels was measured at a specific time and temperature. Hydrogel's swelling ratio (Sr) and percent swelling (Ps) were computed. Results: It was observed that antibiotics were successfully released from the hydrogel throughout the intended 1- week period. The burst effect was relatively minimal, as these profiles demonstrated. The modified triple antibiotic was released sustainably over a long period of time (7 days) in this system owing to biodegradable carrageenan hydrogel. It was also seen that there is minimal degradation associated with carrageenan hydrogel. Conclusion: Our study's findings led us to the conclusion that the modified triple antibiotics' release pattern from hydrogel was acceptable . As a result, carrageenan hydrogel based intracanal medicament can be seen as a novel way to deliver antibiotics to the root canal area and offer localized and prolonged drug release.