In the present study, calculation for turn over frequency and internal and external mass transfer limitations are made for the given kinetics of biodiesel production reaction using Karanja oil as non-edible oil source and Amberlyst15 as heterogeneous acid catalyst. Reaction kinetics are affected by reaction temperature, order of reaction, methanol to oil ratio for Amberlyst15 solid phase catalyst (catalyst active site 4.7 x10-3 mol/g). Weisz-Prater criterion for internal diffusion and Mears criterion for external diffusion are used to predict the mass transfer limitations for different order and temperature of the reaction. There was no mass transfer limitation was found in biodiesel reaction with Amberlyst15 catalyst for different reaction conditions