Enzymes play significant role in food utilization and metabolism. The proteolytic enzymes participate in the breakdown of protein molecules into amino acids and these amino acids are in turn oxidized to give energy for body function. Biochemical analysis of biochemical components, Fish is important to estimate the physiological health of fish along with estimation of water pollution, discussed about the methods and materials to analysis the biochemical components of fish. fish Ctenopharyngodon idella of size 6±7 cm and 6±8 g weight were brought from a local fish farm kuchipudi, Guntur district of Andhra Pradesh, India and acclimatized at 28± 20 C in the laboratory for 15 days. Such acclimatized fish were exposed to sub lethal (1/10th 96 hr LC50) and lethal (96 hr LC 50) concentrations of Phosalone for 24 hr lethal and sub lethal, 4 day 8 days. The vital tissues like muscle, brain, liver, gill and kidney of the fish were taken for the estimation of lactate dehydrogenase (LDH) activity, Estimation of Succinate dehydrogenase (SDH) activity, Malate dehydrogenase (MDH) activity, along with control exposures. Decreased in Enzymes values were observed in tissues of Ctenopharyngodon Idella exposure to Phosalone (35%) EC.