Aim: The aim of the study is to detect Dextrose, which is a simple sugar made from starch. Starch is a naturally occurring complex carbohydrate found in many plants, including corn. In this research, electrochemical detection of dextrose was done from an artificial saliva sample. Materials and methods: Electrochemical detection of dextrose was done from an artificial saliva sample. 0.1 M (2.5 g) Nickel acetate tetrahydrate (C4H14NiO8) and 0.2 M (0.8 M) Sodium hydroxide (NaOH) was dissolved in 100 ml of double distilled water (DD H2O). Co-precipitation method was used. After calcination, the sample was again ground well and stored in a 2 ml vial for further characterization and experiment. 0.1 mM (1 mg) of Direct blue 71 (DB71) was dissolved with 10 ml of DD H2O. Then the dye solution was sonicated in a bath sonicator for 5 mins. 2 mg of NiO was taken and dissolved in a 2 ml of DD H2O and sonicated for 5 mins. Later, 1 ml of DB71 and 1 ml of NiO soln. was mixed in a 2 ml vial and sonicated for 5 mins. Results: Field Emission Scanning Electron Microscopy (FESEM) was used for the modified setup to study the morphology of the materials. Ultra violet (UV) Visible spectroscopy was used to find the wavelength with relation to the absorbance of the sample and to confirm the theoretical assumptions experimentally. From data a graph was plotted for different concentration, and calibrated all the concentration data with respect to the resulting current sensitivity. Conclusion: The use of Nickel Oxide (Nio)/Direct Blue 71 (DB71) Nanoflowers (Nfs) On Glassy Carbon Electrode (GCE) can be used as a method to detect dextrose in artificial saliva.