KuruverKudineer is a Siddha herbal formulation used for treating many disease especially Autism spectrum disorder (Mantha Sanni) for both Children and adults. Dealing with these facts many studies were to be carried out and here Anxiolytic activity is studied in detail. Aim: To evaluate the efficacy of Kuruverkudineer for Anxiolytic activity by using Elevated plus maze method on Mice. Materials and Methods: The pharmacological activity of KuruverKudineer was done in National Institute of siddha, Tambaram Sanatorium after the approval of Institutional Animal ethical committee.