This research aimed to study the efficacy of chia seeds powder (CSP) on eliminating repeatedly heated oil hazards of male rats. Chemical constituents, fatty acid contents, total phenolic, total flavonoid and phenolic components of chia seeds were estimated. Thirty five male albino rats classified into 5 groups. Group 1 fed on basal diet for 8 weeks. Group 2 served as +ve control and fed basal diet containing 4% repeatedly heated sunflower oil in basal diet constituents for 8 weeks. Groups 3 5 as the same of group 2 and fed 2, 4 and 6% chia seeds powder, respectively. Feed intake, body weight gain and feed efficiency ratio were recorded. Serum liver functions, lipid fraction, kidney functions, malondialdehyde and catalase were determined. Results indicated that CSP contained high amounts of fat, fibers, protein, linolenic acid, γ linolenic acid, total phenols, total flavonoids and quercetin. Body weight % of 5th group was higher among all groups followed by 3ed group. Whereas, feed efficiency ratio of 3ed group had the highest mean values followed by 5th group. Remarkable improvement was observed in all groups fed CSP of all tested biochemical parameters. Rats fed 6% CSP showed significant improvement compared with +ve and other tested groups followed by group 4 that fed 4% CSP. According to all above findings CSP may be beneficial in the reduction of harmful side effects of re used oil. So CSP may be useful for human beings